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Kembali| NIM (Student Number) | H1H012048 |
|---|---|
| Nama Mahasiswa | RIZKY ROBYANICA PAING |
| Judul Artikel | ISOLASI GEN PENGKODE PROTEIN CLAUDIN-3 PADA PERMUKAAN TUBUH BENIH GURAME (Oshpronemus gouramy Lac) |
| Abstrak | Protein claudin berperan dalam pembentukan tight junction yang salah satu fungsinya sebagai barier mekanik pada lapisan epithelium. Penelitian ini bertujuan untuk mengisolasi gen pengkode protein claudin-3 pada benih gurame (Osphronemus gouramy) pada permukaan tubuh. Penelitian tentang claudin pada spesies ini baru pertama kali dilakukan. Sampel RNA diisolasi dari jaringan kulit, insang dan saluran pencernaan. Sample RNA digunakan dalam sintesis cDNA yang digunakan sebagai template pada PCR. Amplifikasi gen claudin-3 pada benih gurame (Osphronemus gouramy) telah berhasil dilakukan dan sekuen sepanjang 160 bp dari gen claudin-3 gurame berhasil diketahui. Hasil analisis blast menunjukkan sekuen gen claudin-3 gurame memiliki tingkat similaritas tertinggi dengan gen claudin-3 pada ikan Stegastes partitus dan ikan Larimichthys crocea (92%). Berdasarkan hasil analisis filogenetik gen claudin-3 gurame berada pada cabang yang sama dengan gen claudin-3d dan Claudin-2 pada ikan fugu (Takifugu rubripes). Penelitian lebih lanjut perlu dilakukan untuk mengetahui ekspresi gen claudin-3 pada masing-masing jaringan permukaan tubuh. |
| Abstrak (Inggris) | Claudin proteins is essential part of tight junction that one of its functions is as for mechanical barrier of epithelium. Aim of this study were to isolate gene encoding claudin-3 proteins of giant gourami (Osphronemus gouramy) on body surfaces. This is the first study of claudin gene of the fish. RNA was extracted from skin, gill, and intestine tissues. sample of RNA was reverse-transcribed into cDNA to be used as template in polymerase chain reaction (PCR) method. Claudin-3 gene was successfully amplified from giant gourami fry and a 160 bp partial fragment of the gene was identified. Blast analysis result of the sequence showed that claudin-3 gene of giant gourami was high similar with this of Stegastes partitus and larimichthys crocea (92%). based on phylogenetic analysis result claudin-3 gene from giant gouramy is on same branch with claudin-3d and claudin-2 genes from puffer fish (Takifugu rubripes). Further research is needed to know the expression of cludin-3 gene in each body surface tissues of the fish. |
| Kata Kunci | Claudin-3, Giant fish, Isolation, RNA |
| Nama Pembimbing 1 | Dr. rer. nat. Hamdan Syakuri, S.Pi, M.Si |
| Nama Pembimbing 2 | Emyliana Listiowati, S.Si, M.Sc. |
| Tahun | 2016 |
| Jumlah Halaman | 8 |
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