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Kembali| NIM (Student Number) | G1A009012 |
|---|---|
| Nama Mahasiswa | NOVIA MANTARI |
| Judul Artikel | Perbedaan Jumlah sel Leydig Tikus Putih (Rattus norvegicus) Jantan pada Model Stres Paradoxical Sleep Deprivation, Imobilisasi, dan Footshock Kronik |
| Abstrak | Latar belakang. Stres dapat mempengaruhi sistem reproduksi pria salah satunya sel Leydig. Peningkatan glukokortikoid selama stres diketahui dapat menyebabkan apoptosis sel Leydig. Tujuan penelitian. Mengetahui perbedaan jumlah sel Leydig tikus putih (Rattus norvegicus) jantan pada model stres paradoxical sleep deprivation, imobilisasi, dan footshock kronik. Metode penelitian. Metode eksperimental post test only with control group design dengan 24 ekor tikus putih (Rattus norvegicus) jantan galur Wistar, yang dibagi menjadi 4 kelompok yaitu kelompok I (kelompok kontrol), II (pemberian stres PSD), III (pemberian stres imobilisasi), dan kelompok IV (pemberian stres footshock) dengan lama perlakuan yaitu 25 hari. Parameter yang diperiksa adalah berat badan tikus sebelum dan sesudah perlakuan, massa testis dan jumlah sel Leydig. Analisis data untuk perbedaan berat badan menggunakan Paired T-Test, perbedaan massa testis menggunakan Kruskal-Wallis dan perbedaan jumlah sel Leydig menggunakan analysis of variance (ANOVA) dengan Post Hoc Tukey- LSD. Hasil penelitian. Berat badan tikus meningkat pada kelompok kontrol (p=0,001), massa testis dan jumlah sel Leydig kelompok perlakuan lebih rendah dibandingkan kelompok kontrol, (p=0,006) dan (p<0,001). Kesimpulan. Jumlah sel Leydig tikus putih (Rattus norvegicus) jantan lebih rendah pada model stres paradoxical sleep deprivation, imobilisasi, dan footshock kronik, serta jumlah sel Leydig terendah terdapat pada kelompok paradoxical sleep deprivation. |
| Abstrak (Inggris) | Background. Stress can affect the male reproductive system, one of them is the Leydig cell. Increased glucocorticoid during stress is known to cause apoptosis in Leydig cell. Objective. Knowing difference among the number of Leydig cell in male albino rats (Rattus norvegicus) with various stress model, paradoxical sleep deprivation stress, immobilization stress, and chronic footshock stress. Method. Experimental method with post-test only with control group design. The number of sample was 24 male albino rats (Rattus norvegicus) in Wistar strain, who were divided into 4 groups: group I (control group), group II (PSD stress), group III (immobilization stress), and group IV (footshock stress). The period of treatment was 25 days. Parameters of this study were body weight of rats before and after treatment, testicular mass, and number of Leydig cells. Analysis for the differences in body weight data was using Paired T-test, the difference of testicular mass was using Kruskal-Wallis and the difference number of Leydig cells was using analysis of variance (ANOVA) with Tukey's Post-Hoc LSD. Result. Body weight of rats increased in the control group (p= 0,001), testicular mass and number of Leydig cells lower in the treatment group, (p=0,006) and (p<0,001). Conclusion. The number of Leydig cells of male albino rats (Rattus norvegicus) lower on paradoxical sleep deprivation, immobilization, and chronic footshock stress model, lowest Leydig cell numbers were those given paradoxical sleep deprivation stress model. |
| Kata Kunci | stres, paradoxical sleep deprivation, imobilisasi, footshock, sel Leydig |
| Nama Pembimbing 1 | dr.Fitranto Arjadi, M.Kes |
| Nama Pembimbing 2 | dr.Yudhi Wibowo, M.PH |
| Tahun | 2013 |
| Jumlah Halaman | 11 |
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