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NIM (Student Number)B1J012082
Nama MahasiswaANITA KHAIRUNISA
Judul ArtikelVIABILITAS DAN KARAKTER MORFOLOGI SEL-SEL LIMPA IKAN NILEM (Osteochilus vittatus ) PADA KONDISI IN VITRO
AbstrakGalur sel yang telah tersedia saat ini sebagian besar berasal dari mamalia, sementara pada ikan masih sangat terbatas. Oleh karena itu, perlu dilakukan penelitian sebagai langkah awal dalam pembuaatan galur sel dari ikan, dalam hal ini memanfaatkan jaringan limpa ikan Nilem (Osteochilus vittatus). Limpa pada ikan merupakan salah satu jaringan hematopoitik. Kondisi lingkungan (in vitro) yang sesuai bagi kultur sel-sel suspensi limpa ikan Nilem menjadi tujuan dasar dalam penelitian. Penelitian dilakukan secara eksperimental (RAL Faktorial). Faktor pertama yaitu jenis serum berupa serum ikan Nilem dan Fetal Bovine Serum (FBS). Faktor kedua berupa konsentrasi serum dalam medium yaitu 0%, 5%, 10%. dan 15%. Konfluensi sel, viabilitas dan kepadatan sel dianalisis secara statistik menggunakan ANOVA Two way (kultur primer) dan ANOVA One way (sub-culture) yang dilanjutkan dengan uji Post Hoc, sedangkan karakter morfologi dan proporsi masing-masing tipe sel dianalisis secara deskriptif. Hasil penelitian menunjukkan konfluensi 90% dicapai dalam 7 hari untuk kultur primer dan 6 hari untuk sub-culture. sedangkan nilai viabilitasnya semua perlakuan layak untuk dikultur kembali (≥80%). Hasil kepadatan tertinggi pada kultur primer pada FBS 15%, sedangkan hasil sub-culture pada SI 15%. Hasil pengamatan morfologi sel kultur diperoleh 8 tipe sel dengan karakter berbeda, namun umumnya menyerupai sel darah diantaranya reticulocytes, erythrocytes dan granular anucleate bodies. Tipe sel yang mendominasi > 65% yaitu tipe sel A yang mirip dengan granular anucleate bodies.
Abstrak (Inggris)The a viability of cell strains largely derived from mammals, while the fish is still limited. Therefore, research needs to be done as the first step in making of cell strain of fish, in this case utilize spleen fish Nilem tissue (Osteochilus vittatus). The spleen on the fish is one of the hematopoitic tissue. Environmental conditions (in vitro) for suspension culture cells of the spleen to become the basic purpose Nilem fish research. The research was conducted experimentally using factorial completely randomized design. The first factor was the serum type consisted of fish serum and Fetal Bovine Serum. The second factor was the serum concentration consisted of control (without serum), 5%, 10% and 15%. Four replicates were provided for each treatment. The cell confluence, cell viability, and cell density were analyzed using two ways ANOVA (primary culture) and one way ANOVA (secondary culture), followed by Post Hoc test for cell confluence and cell desity. Cell morphology was describe and the proportion of each cell type was determinate. The results showed that 90% confluence was achieved in 7 days for the primary culture and 6 days for the secondary culture. The cell viability of all experimental groups were ≥ 80% indicating a good culture condition. The highest cell density was observed in primary culture supplemented with 15% Fetal Bovine Serum and in secondary culture supplemented with 15% fish serum. There were 8 different types of cell observed in the primary culture with morphological features similar to the reticulocyte, erythrocyte and granulocyte anucleated bodys. The culture was dominated by granulocyte anucleated bodys (>60%).
Kata Kunciprimary culture, spleen, Nilem, serum, sub-culture
Nama Pembimbing 1Dra. Gratiana. E. Wijayanti, M.Rep.Sc., Ph.D.
Nama Pembimbing 2Endah Sri Palupi, S.Si., M.Sc.
Tahun2012
Jumlah Halaman10
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