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Kembali| NIM (Student Number) | B1A016086 |
|---|---|
| Nama Mahasiswa | ANISA ISTIQOMAH |
| Judul Artikel | Pengaruh Pemberian Bifidobacterium sp. Bb2E Terhadap Kadar Glukosa Darah Pada Mencit Balb/C Hiperglikemia Yang Diinduksi Streptozotocin (STZ) |
| Abstrak | RINGKASAN Diabetes melitus adalah penyakit sindrom metabolik yang ditandai dengan tingginya kadar glukosa darah. Tingginya prevalensi diabetes melitus tipe II dan komplikasinya dapat diatasi dengan probiotik seperti Bifidobacterium sp. Bb2E, sehingga perlu dikaji lebih lanjut manfaat dan pengaruhnya bagi kesehatan. Kesehatan manusia sangat erat kaitannya dengan kondisi sistem pencernaan. Ciri-ciri sistem pencernaan yang baik yaitu memiliki kemampuan dalam mencerna, menyerap, imunitas, dan keseimbangan mikrobiota yang baik. Probiotik di dalam sistem pencernaan mengkolonisasi usus dan menghambat enzim alfa glukosidase dan mencegah glikolisis. Selain itu konsumsi probiotik mampu meningkatkan status antioksidan dalam tubuh yang dapat mencegah komplikasi. Komponen bioaktif probiotik sebagai antioksidan mampu mengurangi radikal bebas dan peroksidase lipid, serta meregenerasi sel β pankreas. Penelitian ini dilakukan untuk mengetahui 1) pengaruh probiotik terhadap perubahan kadar glukosa darah mencit model diabetes, 2) lama pemberian pakan yang efektif dalam mempengaruhi kadar glukosa darah dan korelasi dengan berat badan, jumlah bakteri asam laktat, dan jumlah total bakteri pada feses mencit model diabetes. Penelitian dilakukan secara eksperimental dengan menggunakan Rancangan Acak Kelompok (RAK). Perlakuan hewan uji dibagi menjadi 4 taraf dan masing-masing perlakuan dengan 4 ulangan. Perlakuan 1 (kontrol sehat), mencit tanpa perlakuan. Perlakuan 2 (kontrol sakit), mencit hanya diberi STZ. Perlakuan 3 (kontrol positif), mencit diinduksi STZ dan metformin 45 mg/kg BB. Perlakuan 4, mencit diinduksi STZ dan pakan Bifidobacterium sp. Bb2E terenkapsulasi. Variabel yang digunakan meliputi variabel bebas yaitu lama pemberian pakan dan variabel terikat berupa perubahan kadar glukosa darah. Parameter utama yaitu kadar glukosa darah. Parameter pendukung yaitu berat badan, jumlah bakteri asam laktat pada feses, dan total bakteri pada feses. Data yang diperoleh dianalisis menggunakan Analysis of Varians (ANOVA). Hasil yang signifikan (berbeda nyata) dilanjut dengan uji selang berganda Duncan dengan taraf kepercayaan P < 0,05. Hasil penelitian ini menunjukkan bahwa pemberian Bifidobacterium sp. Bb2E belum efektif menurunkan kadar glukosa darah. Korelasi antara kadar glukosa darah dengan berat badan, jumlah bakteri asam laktat, dan total bakteri pada feses mencit hanya sebesar 26,5%, berarti 73,5% kadar kadar glukosa darah dipengaruhi oleh faktor lain yang tidak terdapat pada penelitian ini. Salah satu faktor yang mempengaruhi kadar glukosa darah yaitu genetik. Kadar glukosa darah pada perlakuan yang diberi pakan Bifidobacterium sp. Bb2E memiliki kadar glukosa darah tertinggi dibandingkan perlakuan lainnya. Lama pemberian pakan Bifidobacterium sp Bb2E selama 5 minggu belum efektif dalam menurunkan kadar glukosa darah mencit hiperglikemia, sehingga perlu dilakukan perpanjangan lama pemberian pakan pada penelitian selanjutnya. |
| Abstrak (Inggris) | SUMMARY Diabetes mellitus was a metabolic syndrome disease characterized by high blood glucose levels. The high prevalence of type II diabetes mellitus and its complications can be overcome with probiotics such as Bifidobacterium sp. Bb2E, so it’s necessary to study further the benefits and effects on health. Human health was closely related to the condition of the digestive system. The characteristics of a good digestive system are good digestion, absorption, immunity and microbiota balance. Probiotics in the digestive system colonize the intestines and inhibit the enzyme alpha glucosidase and prevent glycolysis. In addition, consumption of probiotics can increased the antioxidant status in the body which can prevent complications. The bioactive components of probiotics as antioxidants can reduce free radicals and lipid peroxidase, and regenerate pancreatic β cells. This study aims were conducted to determine 1) the effect of probiotics on changes in blood glucose levels in diabetes model mice, 2) the duration of feeding which was effective influenced blood glucose levels and the correlation with body weight, number of lactic acid bacteria, and total number of bacteria in the feces of mice with diabetes models. This research were conducted experimentally with a randomized block design. The treatment of tested animals were divided into 4 levels and each treatment with 4 replications. Treatment 1 (healthy control), mice without treatment. Treatment 2 (pain control), mice that only given STZ. Treatment 3 (positive control), mice that induced by STZ and metformin 45 mg/kg body weight. Treatment 4, STZ induced mice and Bifidobacterium sp. Bb2E encapsulated. The variables used include independent variables, namely the length of feeding and the dependent variable in the form of changes in blood glucose levels. The main parameter was blood glucose levels, while the supporting parameters were body weight, number of lactic acid bacteria in feces, and total bacteria in feces. The data obtained were analyzed with Analysis of Variance (ANOVA). There were significant results (significantly different), followed Duncan's multiple-interval test with a confidence level of P <0.05. The results of this study showed that administration of Bifidobacterium sp. Bb2E has not been effective in reducing blood glucose levels. The correlation between blood glucose levels and body weight, the number of lactic acid bacteria, and total bacteria in mice feces were only 26.5%, meaning that 73.5% of blood glucose levels were influenced by other factors not present in this study. One of the factor that affected blood glucose levels was genetics. Blood glucose levels in the treatment that has given by Bifidobacterium sp. Bb2E, was the highest blood glucose levels compared to other treatments. The duration of feeding Bifidobacterium sp Bb2E for five weeks has not been effective in reducing blood glucose levels of hyperglycemic mice, so it’s necessary to extend the length of feeding in future studies. |
| Kata Kunci | Bifidobacterium sp., Diabetes Melitus, Metformin, Streptozotocin, Total Plate Count. |
| Nama Pembimbing 1 | Dra. Dyah Fitri Kusharyati, M. P. |
| Nama Pembimbing 2 | Dr. Farida Nur Rachmawati, M. Si. |
| Tahun | 2020 |
| Jumlah Halaman | 52 |
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