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NIM (Student Number)G1A017077
Nama MahasiswaRADYA PUTRA PRATAMA
Judul ArtikelUJI BANDING EFEKTIVITAS BAKTERI ASAM LAKTAT YOGHURT SINGLE STRAIN DAN DOUBLE STRAIN DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli
AbstrakABSTRAK Latar Belakang : Escherichia coli merupakan flora normal pada saluran pencernaan manusia, namun apabila jumlahnya melebihi normal, E. coli akan bersifat patogen sehingga dapat menyebabkan diare. Salah satu upaya untuk menghambat pertumbuhan E. coli adalah dengan mengonsumsi yoghurt. Yoghurt dapat dibagi menjadi yoghurt single strain dan double strain. Tujuan : Penelitian ini bertujuan untuk mengetahui perbandingan efektivitas yoghurt single strain (L. bulgaricus) ; (S. thermophilus) dan double strain (L. bulgaricus dan S. thermophilus) dalam menghambat pertumbuhan E. coli. Metode : Penelitian ini merupakan penelitian Quasi-experiental dengan post test only control group design,dilakukan secara in-vitro dengan metode difusi sumuran. Jumlah perlakuan dalam penelitian ini adalah 13 perlakuan, dibagi menjadi kelompok kontrol, yoghurt single strain (L. bulgaricus) konsentrasi 20%, 40%, 60%, dan 80%, yoghurt single strain (S. thermophilus) konsentrasi 20%, 40%, 60%, dan 80%, dan yoghurt double strain (L. bulgaricus dan S. thermophilus) konsentrasi 20%, 40%, 60%, dan 80%, masing-masing perlakuan terdiri dari 3 sampel. Hasil : Berdasarkan uji parametrik one way anova, terdapat perbedaan bermakna diameter zona hambat yoghurt terhadap E. coli pada minimal dua perlakuan. Dilanjutkan dengan uji post hoc Bonferroni, yoghurt double strain (L. bulgaricus dan S. thermophilus) sudah bisa menghambat pertumbuhan E. coli pada konsentrasi 20%, sedangkan yoghurt single strain (L. bulgaricus) baru mulai menghambat pertumbuhan E. coli pada konsentrasi 40% dan yoghurt single strain (S. thermophilus) baru mulai menghambat pertumbuhan E. coli pada konsentrasi 60%. Kesimpulan : Yoghurt double strain (L. bulgaricus dan S. thermophilus) memiliki efektivitas yang lebih baik dalam menghambat pertumbuhan E. coli dibandingkan yoghurt single strain (L. bulgaricus) ; (S. thermophilus).
Abstrak (Inggris)Background : Escherichia coli is a normal flora in the human digestive tract, but if the amount of E. coli exceeds normal, it will be pathogenic so that it can cause diarrhea. One of the efforts to inhibit the growth of E. coli is by consuming yogurt. Yogurt can be divided into single strain and multiple strain yogurt. Purpopse : This study aimed to determine the effectiveness of single-strain yogurt (L. bulgaricus); (S. thermophilus) and double strain yogurt (L. bulgaricus and S. thermophilus) in inhibiting the growth of E. coli. Method : This research was a Quasi-experiential study with post test only control group design, conducted in-vitro with well diffusion method. The number of treatments in this study were 13 treatments, divided into control groups, single strain yogurt (L. bulgaricus) with concentrations of 20%, 40%, 60%, and 80%, single strain yogurt (S. thermophilus) with concentrations of 20%, 40%, 60%, and 80%, and double strain yoghurt (L. bulgaricus and S. thermophilus) with concentrations of 20%, 40%, 60%, and 80%, each treatment consisted of 3 samples. Result : Based on the one way ANOVA parametric test, there are differences on the diameter of the inhibition zone of yogurt against E. coli in at least two treatments. Followed by Bonferroni's post hoc test, double strain yogurt (L. bulgaricus and S. thermophilus) starts to inhibit the growth of E. coli at a concentration of 20%, while single strain yogurt (L. bulgaricus) begins to inhibit the growth of E. coli at a concentration of 40% and single strain yogurt (S. thermophilus) starts to inhibit the growth of E. coli at a concentration of 60%. Conclusion : Double strain yogurt (L. bulgaricus and S. thermophilus) has a better strength in inhibiting the growth of E. coli than single strain yogurt (L. bulgaricus); (S. thermophilus).
Kata KunciAsam Laktat, Escherichia coli, Lactobacillus bulgaricus, Streptococcus thermophilus, Yoghurt
Nama Pembimbing 1dr. Galuh Yulieta Nitihapsari, M.Biomed
Nama Pembimbing 2dr. Tri Okmawati Handini, M.Si
Tahun2021
Jumlah Halaman13
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