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Kembali| NIM (Student Number) | G1B017020 |
|---|---|
| Nama Mahasiswa | ARINA MANASIKANA ESKA |
| Judul Artikel | PERBEDAAN EKSPRESI GEN MATRIX METALLOPROTEINASE-1 (MMP1) PADA LOW GRADE DAN HIGH GRADE ORAL SQUAMOUS CELL CARCINOMA (Studi pada Pasien Laboratorium Patologi Anatomi RSUD Prof. Dr. Margono Soekarjo Purwokerto) |
| Abstrak | Oral squamous cell carcinoma (OSCC) merupakan keganasan epitel rongga mulut dengan kategori tingkat diferensiasi low dan high grade. MMP1 diketahui berperan pada proses invasi dan metastasis dan ekspresinya meningkat selama progresi kanker. Namun, belum diketahui perubahan ekspresi mRNA MMP1 pada low grade dan high grade pada OSCC. Penelitian ini bertujuan untuk mengetahui perubahan ekspresi mRNA MMP1 pada low dan high grade pasien OSCC. Penelitian observasional analitik dengan rancangan cross sectional menggunakan jaringan FFPE sebanyak 18 sampel OSCC. Sampel FFPE dilakukan ekstraksi RNA dan diubah manjadi bentuk cDNA kemudian kuantifikasi ekspresi mRNA MMP1 menggunakan qPCR. Analisis statistik relatif ekspresi menggunakan software GenEX MultiD untuk mengetahui perbedaan ekspresi low dan high grade dengan uji independent t-test. Hasil analisis statisik menunjukan perubahan ekspresi mRNA MMP1 dengan fold change 12,5 (p<0.05) pada high dibandingkan low grade. Kesimpulan dari penelitian ini, terdapat perubahan kenaikan ekspresi pada mRNA MMP1 pada high dibandingkan low grade. |
| Abstrak (Inggris) | Oral squamous cell carcinoma (OSCC) is an oral epithelial malignancy with low and high grade differentiation categories. MMP1 is known to play a role in the process of invasion and metastasis and its expression increases during cancer progression. However, there is no known change in MMP1 mRNA expression in low grade and high grade OSCC. This study aims to determine changes in MMP1 mRNA expression in low and high grade OSCC patients. Analytical observational study with a cross sectional design using FFPE network as many as 18 samples of OSCC. FFPE samples were extracted with RNA and converted into cDNA form and then quantified MMP1 mRNA expression using qPCR. Statistical analysis of relative expressions used GenEX MultiD software to determine the difference between low and high grade expressions using an independent t-test. The results of statistical analysis showed changes in MMP1 mRNA expression with a fold change of 12.5 (p<0.05) at high compared to low grade. The conclusion of this study, there is a change in the increase in the expression of MMP1 mRNA at high compared to low grade. |
| Kata Kunci | Oral squamous cell carcinoma (OSCC), MMP1, low grade, high grade. |
| Nama Pembimbing 1 | Dr. drg. A Haris Budi Widodo, M.Kes., AP., S.IP., S.E. |
| Nama Pembimbing 2 | dr. Hidayat Sulistyo, Sp.PA., M.Si.Med. |
| Tahun | 2021 |
| Jumlah Halaman | 19 |
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