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NIM (Student Number)D1A018171
Nama MahasiswaFACHMI ANGGA PERMANA
Judul ArtikelAbnormalitas dan Morfometri Spermatozoa Kambing Saanen Hasil Sexing Menggunakan Bovine Serum Albumin dengan Perbandingan Konsentrasi yang Berbeda
AbstrakPenelitian ini bertujuan untuk mengetahui kualitas dan keakuratan hasil sexing dengan mengamati abnormalitas dan morfometri spermatozoa. Penelitian dilakukan dengan menggunakan semen dari seekor ternak pejantan kambing Saanen berumur 2 tahun di Experimental Farm, Universitas Jenderal Soedirman. Metode penelitian ini adalah metode experimental dengan perlakuan perbandingan konsentrasi Bovine Serum Albumin berbeda yang dirancang sebanyak 8 kali ulangan dan 2 perlakuan. Analisis data dilakukan dengan uji beda t student equal dan korelasi pearson. Hasil dari penelitian diperoleh rataan abnormalitas spermatozoa pada P1 konsentrasi 5% = 11,53±5,77% dan 10% = 8,00±3,87% sedangkan pada P2 konsentrasi 15% = 12,79±3,85% dan 20% = 12,27±6,13% dan morfometri spermatozoa pada P1 konsentrasi 5% = 7,88±0,79 μm2 dan 10% = 6,42±0,23 μm2 sedangkan pada P2 konsentrasi 15% = 7,02±0,30 μm2 dan 20% = 6,91±0,40 μm2. Hasil uji beda t student equal menunjukkan abnormalitas spermatozoa pre sexing berpengaruh nyata terhadap abnormalitas hasil sexing. Hasil uji beda t student equal menunjukkan morfometri spermatozoa pre sexing berpengaruh nyata terhadap abnormalitas hasil sexing pada konsentrasi BSA 5% dan 10%, serta morfometri konsentrasi 5% dengan 10%. Hasil uji korelasi pearson menunjukkan adanya korelasi lemah antara ukuran morfometri dengan nilai abnormalitas spermatozo. Kesimpulan yang didapat dari penelitian ini adalah kualitas spermatozoa kambing Saanen hasil sexing yang mendapatkan hasil terbaik yakni semen hasil sexing dengan menggunakan perbandingan Bovine Serum Albumin dengan konsentrasi 5% : 10% berdasarkan peninjauan abnormalitas, keakuratan morfometri dan korelasinya.
Abstrak (Inggris)This study entitled aims to determine the quality and accuracy of sexing results by observing spermatozoa's abnormalities and morphometry. The study was conducted using semen from a 2-year-old Saanen bucks at Experimental Farm, Jenderal Sudirman University. This research method is an experimental method with a comparison of different concentrations of Bovine Serum Albumin which was designed with 8 replications and 2 treatments. Data analysis was carried out by using student equal t-test and Pearson correlation. The results of the study obtained that the average spermatozoa abnormality at P1 concentration of 5% = 11,53±5,77% and 10% = 8,00±3,87% while at P2 concentration of 15% = 12,79±3,85% and 20% = 12,27±6,13% and spermatozoa morphometry at P1 concentration 5% = 7,88±0,79 μm2 and 10% = 6,42±0,23 μm2 while at P2 the concentration of 15% = 7,02±0,30 μm2 and 20% = 6,91±0,40 μm2. The results of the different student equal t test showed that the abnormality of pre-sexed spermatozoa had a significant effect on the abnormality of the sexing results. The results of the different student equal t test showed that the morphometry of pre-sexed spermatozoa significantly affected the abnormality of the sexing results at concentrations of BSA 5% and 10%, and morphometry at concentrations of 5% and 10%. The results of the Pearson correlation test showed a weak correlation between the size of the morphometry and the abnormal value of spermatozoa. The conclusion obtained from this study is the quality of the spermatozoa of Saanen bucks from sexing that get the best results, namely semen from sexing by using a ratio of Bovine Serum Albumin with a concentration of 5%: 10% based on the abnormality, morphometric accuracy and their correlations.
Kata KunciSexing, Bovine Serum Albumin, Morphometry, Abnormality
Nama Pembimbing 1Prof. Ir. Dadang Mulyadi Saleh, M.S., M. Agr. Sc., Ph.D
Nama Pembimbing 2Hermawan Setyo Widodo, S.Pt., M.Si
Tahun2022
Jumlah Halaman9
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